Hotspot

trnN-GUU-rps15

IRb · 99947-101504 · 1558 bp

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Pi 0

MarkerSeek score 58.90

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 1558
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	AGGATTCGAACCTACGACCA	AGCAAGTCTCCGTATCTTATTGG	50.0	43.5	58.2	58.0	1735-1740 bp	1.0	54.6
trnN-GUU-rps15_p2	GGATTCGAACCTACGACCAG	AGCAAGTCTCCGTATCTTATTGG	55.0	43.5	57.8	58.0	1734-1739 bp	1.0	54.2
trnN-GUU-rps15_p3	CCCCAAGTAGGATTCGAACC	AGCAAGTCTCCGTATCTTATTGG	55.0	43.5	57.7	58.0	1743-1748 bp	1.0	53.2
trnN-GUU-rps15_p4	AGGATTCGAACCTACGACCA	GCAAGTCTCCGTATCTTATTGGT	50.0	43.5	58.2	58.3	1734-1739 bp	1.0	52.5
trnN-GUU-rps15_p5	AGGATTCGAACCTACGACCA	GCAAGTCTCCGTATCTTATTGG	50.0	45.5	58.2	56.7	1734-1739 bp	1.0	52.4