Hotspot

ndhC-trnV-UAC

LSC · 49527-50392 · 866 bp

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Pi 0

MarkerSeek score 58.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.0	57.9	976 bp	1.0	79.2
ndhC-trnV-UAC_p2	TCCTTCACTAACCGGTGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.1	57.9	999 bp	1.0	78.8
ndhC-trnV-UAC_p3	ACTAACCGGTGCTAAAAGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.2	57.9	993 bp	1.0	77.9
ndhC-trnV-UAC_p4	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.0	58.3	977 bp	1.0	77.3
ndhC-trnV-UAC_p5	TCCTTCACTAACCGGTGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.1	58.3	1000 bp	1.0	77.0