Hotspot

trnS-GCU-psbD

LSC · 7599-8561 · 963 bp

Back to Arrhenatherum

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1108 bp	1.0	78.3
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	TCCTACAAAAACGAAGCGGT	45.0	45.0	58.0	57.8	1099 bp	1.0	77.7
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1079 bp	1.0	77.2
trnS-GCU-psbD_p4	GAGCGGAAAGAGAGGGATTC	GCCGGACCATCCTACAAAAA	55.0	50.0	57.8	58.2	1159 bp	1.0	76.8
trnS-GCU-psbD_p5	GAGCGGAAAGAGAGGGATTC	TCCTACAAAAACGAAGCGGT	55.0	45.0	57.8	57.8	1150 bp	1.0	76.2