Hotspot

matK-trnK-UUU

LSC · 3307-3970 · 664 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TTCCTGAAAGAGAAGTGGGT	AGAAGCGCCCAGTGTAATTC	45.0	50.0	56.0	58.3	844 bp	1.0	61.7
matK-trnK-UUU_p2	TCCTGAAAGAGAAGTGGGTAGA	AGAAGCGCCCAGTGTAATTC	45.5	50.0	57.9	58.3	843 bp	1.0	60.9
matK-trnK-UUU_p3	TTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCC	45.0	45.0	56.0	57.6	855 bp	1.0	60.4
matK-trnK-UUU_p4	CCTGAAAGAGAAGTGGGTAGAC	AGAAGCGCCCAGTGTAATTC	50.0	50.0	57.8	58.3	842 bp	1.0	60.4
matK-trnK-UUU_p5	TCCTGAAAGAGAAGTGGGTAGA	AATGTTTTGACAGAAGCGCC	45.5	45.0	57.9	57.6	854 bp	1.0	59.6