Hotspot

rps16

LSC · 5409-6546 · 1138 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1138
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGAAACAAAAAGGTTATACAAGGCT TATGTACCGACCGAACCGAT 32.0 50.0 57.4 58.3 1275 bp 1.0 40.0
rps16_p2 AGAAACAAAAAGGTTATACAAGGCT CCGACCGAACCGATGATTAT 32.0 50.0 57.4 57.6 1269 bp 1.0 40.0
rps16_p3 ACAAAAAGGTTATACAAGGCT TATGTACCGACCGAACCGAT 33.3 50.0 53.3 58.3 1271 bp 1.0 40.0
rps16_p4 AGAAACAAAAAGGTTATACAAGGCT ATTATGTACCGACCGAACCG 32.0 50.0 57.4 57.5 1277 bp 1.0 40.0
rps16_p5 AGCAAAGAAACAAAAAGGTT TATGTACCGACCGAACCGAT 30.0 50.0 52.2 58.3 1280 bp 1.0 40.0