Hotspot

rpl20

LSC · 72050-72403 · 354 bp

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Pi 0
MarkerSeek score 28.30
Primer available yes
Label rpl20

Feature Metadata

label_name
rpl20
feature_type
gene
region
LSC
length_bp
354
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20_p1 TCATTCTCCAGGGAACTCCA GACTAGGATACGGGAAGGGA 50.0 55.0 57.7 57.3 481 bp 1.0 71.9
rpl20_p2 TCATTCTCCAGGGAACTCCA AGACTAGGATACGGGAAGGG 50.0 55.0 57.7 57.3 482 bp 1.0 71.8
rpl20_p3 CCAGAGTTCATTCTCCAGGG GACTAGGATACGGGAAGGGA 55.0 55.0 57.3 57.3 488 bp 1.0 69.0
rpl20_p4 CCCAGAGTTCATTCTCCAGG GACTAGGATACGGGAAGGGA 55.0 55.0 57.3 57.3 489 bp 1.0 69.0
rpl20_p5 CCAGAGTTCATTCTCCAGGG AGACTAGGATACGGGAAGGG 55.0 55.0 57.3 57.3 489 bp 1.0 68.9