Hotspot

matK

LSC · 2130-3644 · 1515 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1515
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 CCATTTCTACGATCGCATAACC TGAGTCTTACTTGTTCCGAGG 45.5 47.6 57.4 57.1 1657 bp 1.0 43.3
matK_p2 CCATTTCTACGATCGCATAACC GAGTCTTACTTGTTCCGAGGT 45.5 47.6 57.4 57.1 1656 bp 1.0 43.3
matK_p3 TTTCCATTTCTACGATCGCA TGAGTCTTACTTGTTCCGAGG 40.0 47.6 55.2 57.1 1660 bp 1.0 42.0
matK_p4 TTTCCATTTCTACGATCGCA GAGTCTTACTTGTTCCGAGGT 40.0 47.6 55.2 57.1 1659 bp 1.0 42.0
matK_p5 CCATTTCTACGATCGCATAACC AGTCTTACTTGTTCCGAGGT 45.5 45.0 57.4 55.8 1655 bp 1.0 41.3