Hotspot

rpl20-rps12

LSC · 72404-73173 · 770 bp

Back to Aronia

Pi 0.0013
MarkerSeek score 59.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
770
variable_sites
1
pi
0.0013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 926 bp 1.0 74.9
rpl20-rps12_p2 TTGAATGAGCCCCTCGAAAG AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 925 bp 1.0 74.9
rpl20-rps12_p3 CTTGAATGAGCCCCTCGAAA CAGCGACGAGGAACATGTAC 50.0 55.0 57.9 58.7 889 bp 1.0 73.3
rpl20-rps12_p4 TTGAATGAGCCCCTCGAAAG CAGCGACGAGGAACATGTAC 50.0 55.0 57.9 58.7 888 bp 1.0 73.3
rpl20-rps12_p5 TTGAATGAGCCCCTCGAAAG AGCGACGAGGAACATGTACT 50.0 50.0 57.9 58.8 887 bp 1.0 72.6