Hotspot

matK-trnK-UUU

LSC · 3645-4348 · 704 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
704
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CCATGATCATGAGCAAGTGC AACGGTAGAGTACTCGGCTT 50.0 50.0 57.2 58.2 835 bp 1.0 72.4
matK-trnK-UUU_p2 CCATGATCATGAGCAAGTGC ACGGTAGAGTACTCGGCTTT 50.0 50.0 57.2 58.2 834 bp 1.0 72.4
matK-trnK-UUU_p3 CCATGATCATGAGCAAGTGC TTGGTTTGGATTGCGTTGTG 50.0 45.0 57.2 57.8 930 bp 1.0 72.1
matK-trnK-UUU_p4 CCATGATCATGAGCAAGTGC TTGGGTTGCTAACTCAACGG 50.0 50.0 57.2 58.4 850 bp 1.0 70.6
matK-trnK-UUU_p5 CCATGATCATGAGCAAGTGC GGGTTGCTAACTCAACGGTA 50.0 50.0 57.2 57.5 848 bp 1.0 70.1