primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.107227	232	494	441-441	1.000000	79.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.140446	232	494	391-391	1.000000	78.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.149276	232	494	394-394	1.000000	78.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	232	494	380-380	1.000000	78.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	232	494	394-394	1.000000	78.7
psbA_p1	psbA	1	AGGAGCAATAACAAACCTCTTGA	ATTCATGTGCTTGGGAGTCC	23	20	39.1	50.0	58.0	57.9	0.0	0.0	3.149251	495	1556	1205-1205	1.000000	54.8
psbA_p2	psbA	2	GGAGCAATAACAAACCTCTTGA	ATTCATGTGCTTGGGAGTCC	22	20	40.9	50.0	56.6	57.9	0.0	0.0	3.517585	495	1556	1204-1204	1.000000	51.9
psbA_p3	psbA	3	AGGAGCAATAACAAACCTCTTG	ATTCATGTGCTTGGGAGTCC	22	20	40.9	50.0	56.6	57.9	0.0	0.0	3.526025	495	1556	1205-1205	1.000000	51.8
psbA_p4	psbA	4	AAGGAGCAATAACAAACCTCTTG	ATTCATGTGCTTGGGAGTCC	23	20	39.1	50.0	57.3	57.9	0.0	0.0	3.846941	495	1556	1206-1206	1.000000	49.2
psbA_p5	psbA	5	AGGAGCAATAACAAACCTCT	ATTCATGTGCTTGGGAGTCC	20	20	40.0	50.0	54.2	57.9	0.0	0.0	3.904233	495	1556	1205-1205	1.000000	48.8
matK_p1	matK	1	CCATTTCTACGATCGCATAACC	TGAGTCTTACTTGTTCCGAGG	22	21	45.5	47.6	57.4	57.1	16.6	0.0	4.583663	2130	3644	1657-1657	1.000000	43.3
matK_p2	matK	2	CCATTTCTACGATCGCATAACC	GAGTCTTACTTGTTCCGAGGT	22	21	45.5	47.6	57.4	57.1	16.6	0.0	4.585580	2130	3644	1656-1656	1.000000	43.3
matK_p3	matK	3	TTTCCATTTCTACGATCGCA	TGAGTCTTACTTGTTCCGAGG	20	21	40.0	47.6	55.2	57.1	16.6	0.0	4.745140	2130	3644	1660-1660	1.000000	42.0
matK_p4	matK	4	TTTCCATTTCTACGATCGCA	GAGTCTTACTTGTTCCGAGGT	20	21	40.0	47.6	55.2	57.1	16.6	0.0	4.747057	2130	3644	1659-1659	1.000000	42.0
matK_p5	matK	5	CCATTTCTACGATCGCATAACC	AGTCTTACTTGTTCCGAGGT	22	20	45.5	45.0	57.4	55.8	16.6	0.0	4.831269	2130	3644	1655-1655	1.000000	41.3
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3645	4348	835-835	1.000000	72.4
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3645	4348	834-834	1.000000	72.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCATGATCATGAGCAAGTGC	TTGGTTTGGATTGCGTTGTG	20	20	50.0	45.0	57.2	57.8	32.7	0.0	0.992219	3645	4348	930-930	1.000000	72.1
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCATGATCATGAGCAAGTGC	TTGGGTTGCTAACTCAACGG	20	20	50.0	50.0	57.2	58.4	32.7	9.4	1.176288	3645	4348	850-850	1.000000	70.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.2	57.5	32.7	0.0	1.237069	3645	4348	848-848	1.000000	70.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4386	5408	1095-1095	1.000000	78.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4386	5408	1094-1094	1.000000	78.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.3	58.0	5.3	0.0	0.301348	4386	5408	1104-1104	1.000000	77.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4386	5408	1150-1150	1.000000	77.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4386	5408	1149-1149	1.000000	77.2
rps16_p1	rps16	1	AGAAACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	25	20	32.0	50.0	57.4	58.3	0.0	0.0	5.918149	5409	6546	1275-1275	1.000000	40.0
rps16_p2	rps16	2	AGAAACAAAAAGGTTATACAAGGCT	CCGACCGAACCGATGATTAT	25	20	32.0	50.0	57.4	57.6	0.0	0.0	6.031156	5409	6546	1269-1269	1.000000	40.0
rps16_p3	rps16	3	ACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	21	20	33.3	50.0	53.3	58.3	0.0	0.0	6.032038	5409	6546	1271-1271	1.000000	40.0
rps16_p4	rps16	4	AGAAACAAAAAGGTTATACAAGGCT	ATTATGTACCGACCGAACCG	25	20	32.0	50.0	57.4	57.5	0.0	0.0	6.094395	5409	6546	1277-1277	1.000000	40.0
rps16_p5	rps16	5	AGCAAAGAAACAAAAAGGTT	TATGTACCGACCGAACCGAT	20	20	30.0	50.0	52.2	58.3	0.0	0.0	6.111056	5409	6546	1280-1280	1.000000	40.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6547	7354	887-892	1.000000	78.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	23.3	16.9	0.491095	6547	7354	897-902	1.000000	76.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.6	58.1	0.0	16.9	0.498377	6547	7354	864-869	1.000000	76.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	23.3	16.9	0.499524	6547	7354	898-903	1.000000	76.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	20	20	50.0	55.0	58.2	58.3	0.0	17.9	0.551081	6547	7354	949-954	1.000000	76.0
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.976483	8596	9258	774-774	1.000000	72.2
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.976483	8596	9258	773-773	1.000000	72.2
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	AATCCGACGCTTTAGTCCAC	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.977871	8596	9258	773-773	1.000000	72.2
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AATCCGACGCTTTAGTCCAC	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.977871	8596	9258	772-772	1.000000	72.2
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.2	57.0	0.0	0.0	1.123249	8596	9258	827-827	1.000000	71.0
trnG-GCC_p1	trnG-GCC	1	CAATCCGACGCTTTAGTCCA	CAATGGACGCTTTTCATTCCA	20	21	50.0	42.9	58.0	57.4	0.0	0.0	1.578866	9259	10023	1687-1687	1.000000	67.4
trnG-GCC_p2	trnG-GCC	2	AATCCGACGCTTTAGTCCAC	CAATGGACGCTTTTCATTCCA	20	21	50.0	42.9	58.0	57.4	0.0	0.0	1.580254	9259	10023	1686-1686	1.000000	67.4
trnG-GCC_p3	trnG-GCC	3	CAATCCGACGCTTTAGTCCA	ACAATGGACGCTTTTCATTCC	20	21	50.0	42.9	58.0	57.4	0.0	0.0	1.580548	9259	10023	1688-1688	1.000000	67.4
trnG-GCC_p4	trnG-GCC	4	AATCCGACGCTTTAGTCCAC	ACAATGGACGCTTTTCATTCC	20	21	50.0	42.9	58.0	57.4	0.0	0.0	1.581936	9259	10023	1687-1687	1.000000	67.3
trnG-GCC_p5	trnG-GCC	5	GCCATCTCTCCTCCCAATTG	CAATGGACGCTTTTCATTCCA	20	21	55.0	42.9	58.0	57.4	0.0	0.0	1.595829	9259	10023	1663-1663	1.000000	67.2
rpl20_p1	rpl20	1	TCATTCTCCAGGGAACTCCA	GACTAGGATACGGGAAGGGA	20	20	50.0	55.0	57.7	57.3	0.0	0.0	1.014302	72050	72403	481-481	1.000000	71.9
rpl20_p2	rpl20	2	TCATTCTCCAGGGAACTCCA	AGACTAGGATACGGGAAGGG	20	20	50.0	55.0	57.7	57.3	0.0	0.0	1.021835	72050	72403	482-482	1.000000	71.8
rpl20_p3	rpl20	3	CCAGAGTTCATTCTCCAGGG	GACTAGGATACGGGAAGGGA	20	20	55.0	55.0	57.3	57.3	0.0	0.0	1.378235	72050	72403	488-488	1.000000	69.0
rpl20_p4	rpl20	4	CCCAGAGTTCATTCTCCAGG	GACTAGGATACGGGAAGGGA	20	20	55.0	55.0	57.3	57.3	0.0	0.0	1.378235	72050	72403	489-489	1.000000	69.0
rpl20_p5	rpl20	5	CCAGAGTTCATTCTCCAGGG	AGACTAGGATACGGGAAGGG	20	20	55.0	55.0	57.3	57.3	0.0	0.0	1.385768	72050	72403	489-489	1.000000	68.9
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72404	73173	926-926	1.000000	74.9
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72404	73173	925-925	1.000000	74.9
rpl20-rps12_p3	rpl20-rps12	3	CTTGAATGAGCCCCTCGAAA	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.844694	72404	73173	889-889	1.000000	73.3
rpl20-rps12_p4	rpl20-rps12	4	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.844694	72404	73173	888-888	1.000000	73.3
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	AGCGACGAGGAACATGTACT	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.939077	72404	73173	887-887	1.000000	72.6
ndhF-rpl32_p1	ndhF-rpl32	1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	20	20	40.0	45.0	57.0	57.8	0.0	13.1	1.214860	116442	117522	1277-1277	1.000000	70.3
ndhF-rpl32_p2	ndhF-rpl32	2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	20	20	40.0	45.0	57.0	57.8	0.0	0.0	1.261149	116442	117522	1275-1275	1.000000	70.0
ndhF-rpl32_p3	ndhF-rpl32	3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	20	20	40.0	55.0	57.0	58.3	0.0	0.0	1.377810	116442	117522	1264-1264	1.000000	69.0
ndhF-rpl32_p4	ndhF-rpl32	4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	20	20	40.0	45.0	57.0	57.6	0.0	0.0	1.489633	116442	117522	1274-1274	1.000000	68.1
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	21	20	38.1	45.0	57.7	57.8	0.0	13.1	1.495037	116442	117522	1278-1278	1.000000	68.1