Hotspot

petD

LSC · 73306-74519 · 1214 bp

Back to Arnebia

Pi 0.0211

MarkerSeek score 55.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1214
variable_sites: 46
pi: 0.0211
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TTTTGGGTGTATTGACCGCA	TCGAGGCCAATCAACACAAA	45.0	45.0	58.0	58.0	2163-2216 bp	1.0	84.7
petD_p2	TTTTGGGTGTATTGACCGCA	TTCGAGGCCAATCAACACAA	45.0	45.0	58.0	58.0	2164-2217 bp	1.0	84.7
petD_p3	TTTTGGGTGTATTGACCGCA	TTTCGAGGCCAATCAACACA	45.0	45.0	58.0	58.0	2165-2218 bp	1.0	84.7
petD_p4	TATCGTCCGACCGTTACTGA	TCGAGGCCAATCAACACAAA	50.0	45.0	58.0	58.0	2359-2412 bp	1.0	84.3
petD_p5	TATCGTCCGACCGTTACTGA	TTCGAGGCCAATCAACACAA	50.0	45.0	58.0	58.0	2360-2413 bp	1.0	84.3