Hotspot

accD

LSC · 55768-55877 · 110 bp

Back to Arnebia

Pi 0.163

MarkerSeek score 76.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 110
variable_sites: 40
pi: 0.163
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGGCTACCAAATGGAGTCCT	GACCCACTAAAGGCACCAAA	50.0	50.0	58.0	58.0	1081-2718 bp	1.0	88.9
accD_p2	ACTACAGTTCGGTGGAGGAA	GACCCACTAAAGGCACCAAA	50.0	50.0	58.0	58.0	1224-2861 bp	1.0	88.4
accD_p3	ACTACAGTTCGGTGGAGGAA	TAGACCCACTAAAGGCACCA	50.0	50.0	58.0	58.0	1226-2863 bp	1.0	88.3
accD_p4	ACTACAGTTCGGTGGAGGAA	GGCCGAGACTTTCCATTGAT	50.0	50.0	58.0	58.0	858-862 bp	0.6	73.8
accD_p5	ACTACAGTTCGGTGGAGGAA	CAATAGGGCCGAGACTTTCC	50.0	55.0	58.0	58.0	864-868 bp	0.6	73.8