Hotspot

trnL-UAG-ccsA

SSC · 110027-110128 · 102 bp

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Pi 0.0353
MarkerSeek score 68.30
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
102
variable_sites
7
pi
0.0353
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TGCTCTAAGGTTGAAAATATCA 50.0 31.8 57.9 53.2 186-189 bp 1.0 43.0
trnL-UAG-ccsA_p2 GTAGACACGCTGCTCTTAGG TGCTCTAAGGTTGAAAATATCA 55.0 31.8 57.8 53.2 187-190 bp 1.0 43.0
trnL-UAG-ccsA_p3 AAATTGGTAGACACGCTGCT TGCTCTAAGGTTGAAAATATCA 45.0 31.8 57.8 53.2 193-196 bp 1.0 42.9
trnL-UAG-ccsA_p4 ACAAGCCGCTATGGTGAAAT TGCTCTAAGGTTGAAAATATCA 45.0 31.8 57.9 53.2 209-212 bp 1.0 42.7
trnL-UAG-ccsA_p5 GAGTTGAAGACAAGCCGCTA TGCTCTAAGGTTGAAAATATCA 50.0 31.8 57.9 53.2 218-221 bp 1.0 42.6