Hotspot

rpl32-trnL-UAG

SSC · 109390-109946 · 557 bp

Back to Arnebia

Pi 0.0331

MarkerSeek score 73.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 557
variable_sites: 38
pi: 0.0331
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGTTTTGTTGTGCGACAAACA	TCCTAAGAGCAGCGTGTCTA	38.1	50.0	57.7	57.9	622-648 bp	1.0	76.2
rpl32-trnL-UAG_p2	AGTTTTGTTGTGCGACAAACA	AGCAGCGTGTCTACCAATTT	38.1	45.0	57.7	57.8	615-641 bp	1.0	75.7
rpl32-trnL-UAG_p3	AGTTTTGTTGTGCGACAAACA	CCTAAGAGCAGCGTGTCTAC	38.1	55.0	57.7	57.8	621-647 bp	1.0	75.6
rpl32-trnL-UAG_p4	AGTTTTGTTGTGCGACAAACA	CTAAGAGCAGCGTGTCTACC	38.1	55.0	57.7	57.8	620-646 bp	1.0	75.6
rpl32-trnL-UAG_p5	AGTTTTGTTGTGCGACAAACA	GAGCAGCGTGTCTACCAATT	38.1	50.0	57.7	58.3	616-642 bp	1.0	75.0