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psbE-petL

LSC · 61767-62933 · 1167 bp

Back to Arnebia

Pi 0.0232

MarkerSeek score 68.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1167
variable_sites: 51
pi: 0.0232
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1202-1235 bp	1.0	86.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1204-1237 bp	1.0	86.1
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GCCGCTAGTAGAAAACCGAA	55.0	50.0	58.2	58.0	1197-1230 bp	1.0	85.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AAGCCGCTAGTAGAAAACCG	55.0	50.0	58.2	58.0	1199-1232 bp	1.0	85.7
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.4	58.0	1195-1228 bp	1.0	84.6