Hotspot

petA-psbJ

LSC · 60171-60983 · 813 bp

Back to Arnebia

Pi 0.027

MarkerSeek score 73.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 47
pi: 0.027
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	923-941 bp	1.0	82.9
petA-psbJ_p2	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	938-956 bp	1.0	82.6
petA-psbJ_p3	ACCCATTACGTGTTCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.5	57.6	940-958 bp	1.0	80.2
petA-psbJ_p4	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	933-951 bp	1.0	79.8
petA-psbJ_p5	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	932-950 bp	1.0	79.4