Hotspot

trnM-CAU-atpE

LSC · 50956-51207 · 252 bp

Back to Arnebia

Pi 0.0344

MarkerSeek score 68.60

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 19
pi: 0.0344
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GCCTCTGGCTTAGATCAACC	CCCTATTGCCACAGCTGTAG	55.0	55.0	58.0	58.0	1192-1221 bp	1.0	83.5
trnM-CAU-atpE_p2	TCTCAAATCGAGGATGGGGT	CCCTATTGCCACAGCTGTAG	50.0	55.0	58.1	58.0	1019-1049 bp	1.0	83.2
trnM-CAU-atpE_p3	GCCTCTGGCTTAGATCAACC	GCGCAAAGGGTAAAGGAAAC	55.0	50.0	58.0	57.9	1637-1666 bp	1.0	82.3
trnM-CAU-atpE_p4	GCCTCTGGCTTAGATCAACC	GATCCACAAGAAGCTCAGCA	55.0	50.0	58.0	57.9	1044-1073 bp	0.6	66.1
trnM-CAU-atpE_p5	GCCTCTGGCTTAGATCAACC	TGATCCACAAGAAGCTCAGC	55.0	50.0	58.0	57.9	1045-1074 bp	0.6	66.1