Hotspot

trnF-GAA-ndhJ

LSC · 47463-48103 · 641 bp

Back to Arnebia

Pi 0.0191

MarkerSeek score 70.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 24
pi: 0.0191
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	804-833 bp	1.0	83.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GATAGGATGGCCCCTACGTA	55.0	55.0	58.0	57.8	764-793 bp	1.0	83.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	50.0	45.0	58.1	58.2	768-797 bp	1.0	83.4
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCCTA	55.0	55.0	58.0	58.3	768-797 bp	1.0	83.3
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCCCTAC	55.0	55.0	58.0	58.3	767-796 bp	1.0	83.3