Hotspot

trnG-UCC-trnR-UCU

LSC · 9075-9250 · 176 bp

Back to Arnebia

Pi 0.0375
MarkerSeek score 80.90
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
176
variable_sites
15
pi
0.0375
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG TGGAAACAAATTGCGTCCAA 50.0 40.0 58.0 56.7 303-309 bp 1.0 77.0
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG TGGAAACAAATTGCGTCCAA 55.0 40.0 58.1 56.7 306-312 bp 1.0 76.7
trnG-UCC-trnR-UCU_p3 CCCTAGCCTTCCAAGCTAAC TGGAAACAAATTGCGTCCAA 55.0 40.0 57.7 56.7 307-313 bp 1.0 74.5
trnG-UCC-trnR-UCU_p4 CTAACGATGCGGGTTCGATT TGGAAACAAATTGCGTCCAA 50.0 40.0 58.4 56.7 292-298 bp 1.0 74.1
trnG-UCC-trnR-UCU_p5 TAACGATGCGGGTTCGATTC TGGAAACAAATTGCGTCCAA 50.0 40.0 58.4 56.7 291-297 bp 1.0 74.1