Hotspot

rps16-trnQ-UUG

LSC · 6002-6340 · 339 bp

Back to Arnebia

Pi 0.02
MarkerSeek score 51.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
339
variable_sites
12
pi
0.02
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 408-438 bp 1.0 83.9
rps16-trnQ-UUG_p2 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 410-440 bp 1.0 82.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 401-431 bp 1.0 82.9
rps16-trnQ-UUG_p4 CATGTCTTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 411-441 bp 1.0 82.2
rps16-trnQ-UUG_p5 CCACAGCGGATCATGTCTTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 422-452 bp 1.0 81.8