Hotspot

trnK-UUU-rps16

LSC · 4199-4878 · 680 bp

Back to Arnebia

Pi 0.0275
MarkerSeek score 68.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
680
variable_sites
39
pi
0.0275
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1494-1517 bp 1.0 84.4
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1493-1516 bp 1.0 84.4
trnK-UUU-rps16_p3 TGAAGCCCTAGAGCCCTATC AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1639-1662 bp 1.0 84.1
trnK-UUU-rps16_p4 TCCCTTTCAGGATCAGTCGT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1495-1518 bp 1.0 84.0
trnK-UUU-rps16_p5 TCAGGATCAGTCGTGGTCTT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1489-1512 bp 1.0 83.9