Hotspot

trnH-GUG-psbA

LSC · 97-395 · 299 bp

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Pi 0.0955

MarkerSeek score 81.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 299
variable_sites: 52
pi: 0.0955
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGACTTAGCTGCTGTCGAAG	55.0	50.0	58.1	57.4	252-366 bp	1.0	85.8
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AGACTTAGCTGCTGTCGAAG	50.0	50.0	57.9	57.4	266-380 bp	1.0	85.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AGACTTAGCTGCTGTCGAAG	50.0	50.0	57.9	57.4	265-379 bp	1.0	85.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGACTTAGCTGCTGTCGAAG	50.0	50.0	57.9	57.4	267-381 bp	1.0	85.8
trnH-GUG-psbA_p5	GGAATCACAATCCACTGCCT	AGACTTAGCTGCTGTCGAAG	50.0	50.0	57.9	57.4	273-387 bp	1.0	85.8