Hotspot

ycf4-cemA

LSC · 65161-66011 · 851 bp

Back to Aristotelia

Pi 0.0733

MarkerSeek score 81.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 50
pi: 0.0733
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.9	58.3	917-954 bp	1.0	85.2
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAGAGAGAGATCCACCAGGG	50.0	60.0	58.1	58.3	919-956 bp	1.0	85.0
ycf4-cemA_p3	TGCGCGTACCAATTGAAGTA	TGAGAGAGAGATCCACCAGG	45.0	55.0	57.9	57.5	918-955 bp	1.0	84.0
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	TGAGAGAGAGATCCACCAGG	50.0	55.0	58.1	57.5	920-957 bp	1.0	83.7
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.7	58.3	922-959 bp	1.0	83.3