Hotspot

psbZ-trnG-GCC

LSC · 39594-40281 · 688 bp

Back to Aristotelia

Pi 0.157

MarkerSeek score 77.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 67
pi: 0.157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGGATTGGATTAGTCTTTCTGGT	AATCGAACCCGCATCTTCTC	39.1	50.0	57.2	58.1	549-797 bp	1.0	61.3
psbZ-trnG-GCC_p2	TGGATTGGATTAGTCTTTCTGGT	GAATCGAACCCGCATCTTCT	39.1	50.0	57.2	58.1	550-798 bp	1.0	61.2
psbZ-trnG-GCC_p3	TGGATTAGTCTTTCTGGTAGGT	AATCGAACCCGCATCTTCTC	40.9	50.0	55.9	58.1	544-792 bp	1.0	58.8
psbZ-trnG-GCC_p4	TGGATTAGTCTTTCTGGTAGGT	GAATCGAACCCGCATCTTCT	40.9	50.0	55.9	58.1	545-793 bp	1.0	58.8
psbZ-trnG-GCC_p5	AGTCTTTCTGGTAGGTATCCT	AATCGAACCCGCATCTTCTC	42.9	50.0	54.8	58.1	538-786 bp	1.0	58.0