Hotspot

psbM-trnD-GUC

LSC · 32107-33240 · 1134 bp

Back to Aristotelia

Pi 0.0675

MarkerSeek score 80.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 62
pi: 0.0675
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATTGGTCAGAGCACCG	39.1	50.0	57.6	58.5	1049-1251 bp	1.0	57.0
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	40.0	50.0	54.5	58.5	1043-1245 bp	1.0	56.6
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	39.1	50.0	57.4	58.5	1046-1248 bp	1.0	55.6
psbM-trnD-GUC_p4	AGGAACTAAAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	39.1	50.0	57.3	58.5	1050-1252 bp	1.0	54.9
psbM-trnD-GUC_p5	AGGAACTAAAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	36.4	50.0	56.2	58.5	1050-1252 bp	1.0	54.0