Hotspot

atpH-atpI

LSC · 15452-16364 · 913 bp

Back to Aristotelia

Pi 0.0556

MarkerSeek score 89.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 913
variable_sites: 48
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 89.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1001-1237 bp	1.0	82.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	974-1210 bp	1.0	82.1
atpH-atpI_p3	CGCGATTCCTTCTACAGCTT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.1	58.5	1089-1325 bp	1.0	81.7
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1057-1293 bp	1.0	81.6
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1039-1275 bp	1.0	81.5