Hotspot

rps16-trnQ-UUG

LSC · 6348-8207 · 1860 bp

Back to Aristotelia

Pi 0.141
MarkerSeek score 70.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1860
variable_sites
92
pi
0.141
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.35
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 745-1951 bp 1.0 88.6
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 743-1949 bp 1.0 88.6
rps16-trnQ-UUG_p3 GATGTAAGAGTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 759-1965 bp 1.0 88.2
rps16-trnQ-UUG_p4 GGATGTAAGAGTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 760-1966 bp 1.0 88.2
rps16-trnQ-UUG_p5 ACACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.7 58.1 722-1928 bp 1.0 86.4