Hotspot

trnK-UUU-rps16

LSC · 4320-5170 · 851 bp

Back to Aristotelia

Pi 0.139

MarkerSeek score 81.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 100
pi: 0.139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	CCCAATGGGCCATTTATCGA	55.0	50.0	58.0	58.0	1216-1303 bp	1.0	90.4
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	TCCCAATGGGCCATTTATCG	55.0	50.0	58.0	58.0	1217-1304 bp	1.0	90.4
trnK-UUU-rps16_p3	GACGGTATGGACGAAGCATT	CCCAATGGGCCATTTATCGA	50.0	50.0	58.1	58.0	1180-1267 bp	1.0	90.2
trnK-UUU-rps16_p4	GACGGTATGGACGAAGCATT	TCCCAATGGGCCATTTATCG	50.0	50.0	58.1	58.0	1181-1268 bp	1.0	90.2
trnK-UUU-rps16_p5	TCAGGATCAGTCGTGGTCTT	CCCAATGGGCCATTTATCGA	50.0	50.0	58.1	58.0	1212-1299 bp	1.0	89.9