Hotspot

rpl32-trnL-UAG

SSC · 117243-117873 · 631 bp

Back to Aristolochia

Pi 0.0386

MarkerSeek score 75.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 631
variable_sites: 133
pi: 0.0386
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGCAGTTCCAAAGAAACGT	CTCTGGCACTGCTTCCTAAG	45.0	55.0	58.2	58.0	825-1052 bp	1.0	89.9
rpl32-trnL-UAG_p2	TGGCAGTTCCAAAGAAACGT	CTGGCACTGCTTCCTAAGAG	45.0	55.0	58.2	58.0	823-1050 bp	1.0	89.9
rpl32-trnL-UAG_p3	TGGCAGTTCCAAAGAAACGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	812-1039 bp	1.0	89.1
rpl32-trnL-UAG_p4	TGGCAGTTCCAAAGAAACGT	AGTTAAAAGCCCCGAAGTGG	45.0	50.0	58.2	58.1	1304-1552 bp	0.897436	85.2
rpl32-trnL-UAG_p5	TGGCAGTTCCAAAGAAACGT	TTTAGAGAATGCCGCCACTC	45.0	50.0	58.2	58.0	865-966 bp	0.794872	79.5