Hotspot

trnF-GAA-ndhJ

LSC · 50964-51716 · 753 bp

Back to Aristolochia

Pi 0.0372

MarkerSeek score 78.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 141
pi: 0.0372
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TCTTGATGCCCGAAAGTTGG	55.0	50.0	58.0	58.5	872-932 bp	1.0	88.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TCTTGATGCCCGAAAGTTGG	50.0	50.0	58.1	58.5	836-896 bp	1.0	87.9
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	TCTTGATGCCCGAAAGTTGG	50.0	50.0	57.9	58.5	881-941 bp	1.0	87.6
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	TCTTGATGCCCGAAAGTTGG	55.0	50.0	57.9	58.5	860-920 bp	1.0	87.2
trnF-GAA-ndhJ_p5	TCATGCATCGAGAATGGTCG	TCTTGATGCCCGAAAGTTGG	50.0	50.0	58.1	58.5	891-951 bp	1.0	87.1