Hotspot

ycf3-trnS-GGA

LSC · 46742-47648 · 907 bp

Back to Aristolochia

Pi 0.0333

MarkerSeek score 75.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 160
pi: 0.0333
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCACTTATCCGAGATCTAGGC	CTACGCCTTGAACCACTCG	52.4	57.9	57.1	58.3	946-1020 bp	1.0	67.0
ycf3-trnS-GGA_p2	CCACTTATCCGAGATCTAGGC	GCTACGCCTTGAACCACTC	52.4	57.9	57.1	58.5	947-1021 bp	1.0	64.8
ycf3-trnS-GGA_p3	CACTTATCCGAGATCTAGGCA	CTACGCCTTGAACCACTCG	47.6	57.9	56.4	58.3	945-1019 bp	1.0	61.3
ycf3-trnS-GGA_p4	TCCACTTATCCGAGATCTAGGC	CTACGCCTTGAACCACTCG	50.0	57.9	58.6	58.3	947-1021 bp	1.0	61.3
ycf3-trnS-GGA_p5	CCACTTATCCGAGATCTAGGC	GTGCTACGCCTTGAACCAC	52.4	57.9	57.1	59.1	949-1023 bp	1.0	60.1