Hotspot

trnC-GCA-petN

LSC · 28366-29553 · 1188 bp

Back to Aristolochia

Pi 0.0389

MarkerSeek score 82.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 249
pi: 0.0389
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATCCCTTTCTCCCCAGTTCA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	678-1356 bp	1.0	90.3
trnC-GCA-petN_p2	ATCCCTTTCTCCCCAGTTCA	AGACCACCATTAAAGCAGCC	50.0	50.0	57.9	58.2	694-1372 bp	1.0	90.2
trnC-GCA-petN_p3	TCCCTTTCTCCCCAGTTCAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	677-1355 bp	1.0	89.4
trnC-GCA-petN_p4	TCCCTTTCTCCCCAGTTCAA	AGACCACCATTAAAGCAGCC	50.0	50.0	58.2	58.2	693-1371 bp	1.0	89.3
trnC-GCA-petN_p5	ACTGCAAATCCCTTTCTCCC	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	685-1363 bp	1.0	89.0