Hotspot

atpF-atpH

LSC · 12950-13489 · 540 bp

Back to Aristolochia

Pi 0.0464

MarkerSeek score 76.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 540
variable_sites: 120
pi: 0.0464
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GAGTACCTTCTTGTGCCTCG	TGATTTCTGCCGCTTCTGTT	55.0	45.0	58.0	58.1	753-1215 bp	1.0	91.2
atpF-atpH_p2	GAGTACCTTCTTGTGCCTCG	GCCAAGCCGTAGAAGGTATC	55.0	55.0	58.0	58.1	752-1128 bp	0.948718	88.9
atpF-atpH_p3	GAGTACCTTCTTGTGCCTCG	GGTGTGAGGAGCTTACCATG	55.0	55.0	58.0	58.0	753-1242 bp	0.794872	82.1
atpF-atpH_p4	GAGTACCTTCTTGTGCCTCG	CGGGTGGATGGGTCTTAATG	55.0	55.0	58.0	58.0	753-1320 bp	0.717949	78.6
atpF-atpH_p5	GAGTACCTTCTTGTGCCTCG	TCGGGTGGATGGGTCTTAAT	55.0	50.0	58.0	58.1	753-1321 bp	0.717949	78.0