Hotspot

trnS-GCU-trnG-UCC

LSC · 7944-8816 · 873 bp

Back to Aristolochia

Pi 0.0597

MarkerSeek score 82.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 873
variable_sites: 237
pi: 0.0597
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	TACCACTAAACTATACCCGC	50.0	45.0	57.6	54.0	695-1032 bp	1.0	56.6
trnS-GCU-trnG-UCC_p2	GCTTTAGTCCACTCAGCCAT	TTACCACTAAACTATACCCGC	50.0	42.9	57.6	54.9	696-1033 bp	1.0	55.5
trnS-GCU-trnG-UCC_p3	AGTCCACTCAGCCATCTCTC	TACCACTAAACTATACCCGC	55.0	45.0	58.5	54.0	690-1027 bp	0.974359	54.8
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	TTTACCACTAAACTATACCCGC	50.0	40.9	57.6	55.6	697-1034 bp	1.0	53.8
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	TTACCACTAAACTATACCCGC	55.0	42.9	58.5	54.9	691-1028 bp	0.974359	53.7