Hotspot

rps16-trnQ-UUG

LSC · 6048-6592 · 545 bp

Back to Aristolochia

Pi 0.0644
MarkerSeek score 80.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
545
variable_sites
154
pi
0.0644
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 465-728 bp 1.0 85.1
rps16-trnQ-UUG_p2 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.2 57.3 469-732 bp 1.0 80.4
rps16-trnQ-UUG_p3 CACGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 47.6 55.0 57.5 58.1 467-730 bp 1.0 79.7
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GGTTTTGGTCCCGTTATTCG 50.0 50.0 57.2 57.1 485-748 bp 1.0 78.5
rps16-trnQ-UUG_p5 CACGTTGCTTTCTACCACAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 56.4 58.1 467-730 bp 1.0 78.3