Hotspot

trnK-UUU-rps16

LSC · 4178-4940 · 763 bp

Back to Aristolochia

Pi 0.0804
MarkerSeek score 84.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
763
variable_sites
251
pi
0.0804
unique_haplotype_count
38
primer_available
yes
alignment_reliability
0.96
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TAGTCGCACTTAAAAGCCGA TCGAGTTAATCAAACAAAGTCCA 45.0 34.8 57.3 56.4 755-1292 bp 1.0 52.0
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG TCGAGTTAATCAAACAAAGTCCA 50.0 34.8 58.6 56.4 754-1291 bp 0.974359 51.0
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA TCGAGTTAATCAAACAAAGTCCA 45.0 34.8 57.3 56.4 752-1289 bp 0.974359 51.0
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC TCGAGTTAATCAAACAAAGTCCA 50.0 34.8 57.2 56.4 751-1288 bp 0.974359 51.0
trnK-UUU-rps16_p5 GTCGCACTTAAAAGCCGAGT TCGAGTTAATCAAACAAAGTCCA 50.0 34.8 58.9 56.4 753-1290 bp 0.974359 51.0