Hotspot

psbA-trnK-UUU

LSC · 1190-1484 · 295 bp

Back to Aristolochia

Pi 0.0464

MarkerSeek score 76.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 82
pi: 0.0464
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GTGCTTTCGCGTCTCTCTAA	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.0	58.6	364-411 bp	1.0	87.0
psbA-trnK-UUU_p2	CATCAAAACGCCGAACCATC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.1	58.6	443-490 bp	1.0	86.3
psbA-trnK-UUU_p3	AAACGCCGAACCATCCAATA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.9	58.6	438-485 bp	1.0	86.2
psbA-trnK-UUU_p4	AAAGACGGTTCTCAGTGCTG	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.1	58.6	417-464 bp	1.0	86.1
psbA-trnK-UUU_p5	GATCATCAAAACGCCGAACC	ACTCCATCCGACTAGTTCCG	50.0	55.0	57.8	58.6	446-493 bp	1.0	85.7