Hotspot

petB

LSC · 70863-72322 · 1460 bp

Back to Aristida

Pi 0.0104

MarkerSeek score 57.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1460
variable_sites: 43
pi: 0.0104
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	ACCAGATATGGAGATGGCGA	TGCTCAATATCCAAGTGGGC	50.0	50.0	58.0	57.9	2596-2657 bp	1.0	84.4
petB_p2	TGGCTACACAAACCGTTGAA	TGCTCAATATCCAAGTGGGC	45.0	50.0	58.0	57.9	2453-2514 bp	1.0	84.4
petB_p3	GAACTGGCGCAGGTAGTTTA	TGCTCAATATCCAAGTGGGC	50.0	50.0	57.9	57.9	2408-2469 bp	1.0	84.1
petB_p4	TTTATGGGGGTCACAATGGC	TGCTCAATATCCAAGTGGGC	50.0	50.0	58.1	57.9	2331-2392 bp	1.0	83.9
petB_p5	ACCAGATATGGAGATGGCGA	GTTGGCGTTTATCCCCTCAA	50.0	50.0	58.0	58.2	2528-2591 bp	1.0	83.6