Hotspot

ndhF

SSC · 102788-105004 · 2217 bp

Back to Aristida

Pi 0.0112

MarkerSeek score 53.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 75
pi: 0.0112
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAGGAATTCATGGAATAATGA	AGGTTTGGTGAATCGGAAACA	31.8	42.9	52.9	58.1	2308-2342 bp	1.0	43.1
ndhF_p2	TGAGGAATTCATGGAATAATGAA	AGGTTTGGTGAATCGGAAACA	30.4	42.9	53.8	58.1	2308-2342 bp	1.0	43.1
ndhF_p3	TTGAGGAATTCATGGAATAATGA	AGGTTTGGTGAATCGGAAACA	30.4	42.9	53.8	58.1	2309-2343 bp	1.0	43.1
ndhF_p4	TTGAGGAATTCATGGAATAATGAA	AGGTTTGGTGAATCGGAAACA	29.2	42.9	54.5	58.1	2309-2343 bp	1.0	43.1
ndhF_p5	TTTGAGGAATTCATGGAATAATGA	AGGTTTGGTGAATCGGAAACA	29.2	42.9	54.5	58.1	2310-2344 bp	1.0	43.1