Hotspot

atpB-rbcL

LSC · 53756-54529 · 774 bp

Back to Aristida

Pi 0.0107

MarkerSeek score 66.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 26
pi: 0.0107
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	922-936 bp	1.0	83.5
atpB-rbcL_p2	TATCCAGCACGGGTCCAATA	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.2	58.1	886-900 bp	1.0	83.3
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	919-933 bp	1.0	83.2
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.2	58.1	883-897 bp	1.0	83.1
atpB-rbcL_p5	TATCCAGCACGGGTCCAATA	TGGTGACATAAGTCCCTCCC	50.0	55.0	58.2	58.4	887-901 bp	1.0	80.9