Hotspot

ndhC-trnV-UAC

LSC · 50228-50807 · 580 bp

Back to Aristida

Pi 0.0184
MarkerSeek score 71.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
580
variable_sites
32
pi
0.0184
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCTAAAAGCCCTGAAATCCA GGTTCGAATCCGTATAGCCC 45.0 55.0 56.0 57.9 679-725 bp 1.0 71.0
ndhC-trnV-UAC_p2 AGCCCTGAAATCCAAAATACCA GGTTCGAATCCGTATAGCCC 40.9 55.0 57.9 57.9 673-719 bp 1.0 70.6
ndhC-trnV-UAC_p3 GCTAAAAGCCCTGAAATCCA AGCCCAATGCAGAAAAATGC 45.0 45.0 56.0 57.9 772-818 bp 1.0 70.3
ndhC-trnV-UAC_p4 AGCCCTGAAATCCAAAATACCA AGCCCAATGCAGAAAAATGC 40.9 45.0 57.9 57.9 766-812 bp 1.0 69.9
ndhC-trnV-UAC_p5 GCTAAAAGCCCTGAAATCCAA GGTTCGAATCCGTATAGCCC 42.9 55.0 56.8 57.9 679-725 bp 1.0 69.2