Hotspot

trnT-UGU-trnL-UAA

LSC · 46188-46958 · 771 bp

Back to Aristida

Pi 0.0218

MarkerSeek score 66.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 47
pi: 0.0218
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	866-910 bp	1.0	87.7
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	858-902 bp	1.0	87.5
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	836-880 bp	1.0	87.1
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	GCTTCCATTGAGTCTCTGCA	50.0	50.0	58.0	57.9	1030-1074 bp	1.0	86.0
trnT-UGU-trnL-UAA_p5	TCCTCGATAACGGGACATGA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	57.9	58.0	1205-1265 bp	0.875	79.3