Hotspot

atpI-atpH

LSC · 31905-32634 · 730 bp

Back to Aristida

Pi 0.0127

MarkerSeek score 64.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 28
pi: 0.0127
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGG	AAGCAGCAGCAATTAGTGGA	55.0	45.0	58.2	57.8	741-901 bp	1.0	83.2
atpI-atpH_p2	TAGCCGCAGCCTATATAGGG	GCAGTACCTTGACCAACTCC	55.0	55.0	58.2	58.2	808-968 bp	1.0	83.1
atpI-atpH_p3	TAGCCGCAGCCTATATAGGG	GCCAATCCAGCAGCAATAAC	55.0	50.0	58.2	57.8	763-923 bp	1.0	83.1
atpI-atpH_p4	TAGCCGCAGCCTATATAGGG	ATAACGGAAGCAGCAGCAAT	55.0	45.0	58.2	58.2	748-908 bp	1.0	82.9
atpI-atpH_p5	TAGCCGCAGCCTATATAGGG	TCCAGCAGCAATAACGGAAG	55.0	50.0	58.2	58.3	758-918 bp	1.0	82.7