Hotspot

psbM-petN

LSC · 17148-17598 · 451 bp

Back to Aristida

Pi 0.0212

MarkerSeek score 62.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 25
pi: 0.0212
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGGATGTCTATGTGACCCGT	CGGATTCTGTCGAGAACCAG	50.0	55.0	58.1	58.1	1358-1505 bp	1.0	86.0
psbM-petN_p2	TGGATGTCTATGTGACCCGT	TCTCTCTATTCCCGCGTCTT	50.0	50.0	58.1	57.9	1258-1276 bp	1.0	83.2
psbM-petN_p3	TGGATGTCTATGTGACCCGT	TCTCTATTCCCGCGTCTTCT	50.0	50.0	58.1	57.9	1256-1274 bp	1.0	83.1
psbM-petN_p4	TGGATGTCTATGTGACCCGT	TAGGGCCGAATCACCTTTTG	50.0	50.0	58.1	57.9	1099-1117 bp	0.875	77.1
psbM-petN_p5	TGGATGTCTATGTGACCCGT	GGACATCCTCCTACCCCTTT	50.0	55.0	58.1	58.1	1231-1249 bp	0.875	76.7