Hotspot

trnT-GGU-trnE-UUC

LSC · 14879-15357 · 479 bp

Back to Aristida

Pi 0.0129
MarkerSeek score 65.00
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
479
variable_sites
21
pi
0.0129
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGGCATAAGTCATCGGTTCA TGTCCTGAACCACTAGACGA 45.0 50.0 56.6 57.7 538-836 bp 1.0 72.8
trnT-GGU-trnE-UUC_p2 AGCCCTTTTAACTCAGTGGT TGTCCTGAACCACTAGACGA 45.0 50.0 56.7 57.7 575-836 bp 1.0 72.7
trnT-GGU-trnE-UUC_p3 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.6 58.3 559-857 bp 1.0 72.7
trnT-GGU-trnE-UUC_p4 AGCCCTTTTAACTCAGTGGT GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.7 58.3 596-857 bp 1.0 72.6
trnT-GGU-trnE-UUC_p5 TGGTAAGGCATAAGTCATCGG GCTGCCTCCTTGAAAGAGAG 47.6 55.0 57.5 58.3 564-856 bp 1.0 71.9