Hotspot

trnG-UCC-trnT-GGU

LSC · 14152-14806 · 655 bp

Back to Aristida

Pi 0.025

MarkerSeek score 75.20

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 47
pi: 0.025
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	402-793 bp	1.0	50.0
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	402-789 bp	1.0	49.3
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	403-790 bp	1.0	49.3
trnG-UCC-trnT-GGU_p4	AGAACGAATCACACTTTTACCAC	TGAACCGATGACTTATGCCT	39.1	45.0	57.3	56.6	405-796 bp	1.0	49.3
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	39.1	45.0	57.3	56.6	404-795 bp	1.0	49.3