Hotspot

ndhG-ndhI

SSC · 132817-133514 · 698 bp

Back to Arisaema

Pi 0.123

MarkerSeek score 77.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 698
variable_sites: 259
pi: 0.123
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGTAACCCCATCTCCCACA	CTACCCGTTGTGGATTGGAG	50.0	55.0	57.9	58.0	930-1519 bp	1.0	91.4
ndhG-ndhI_p2	CAGCGAAGTAACCCCATCTC	CTACCCGTTGTGGATTGGAG	55.0	55.0	58.1	58.0	935-1524 bp	1.0	91.3
ndhG-ndhI_p3	CCATCTCCCACAGTCCAAAG	CTACCCGTTGTGGATTGGAG	55.0	55.0	57.9	58.0	922-1511 bp	1.0	90.8
ndhG-ndhI_p4	AAGTAACCCCATCTCCCACA	CCCGTTGTGGATTGGAGATT	50.0	50.0	57.9	57.9	927-1516 bp	1.0	90.5
ndhG-ndhI_p5	CAGCGAAGTAACCCCATCTC	CCCGTTGTGGATTGGAGATT	55.0	50.0	58.1	57.9	932-1521 bp	1.0	90.5