Hotspot

ndhE-ndhG

SSC · 131319-132285 · 967 bp

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Pi 0.0934

MarkerSeek score 79.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 967
variable_sites: 237
pi: 0.0934
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GTGCTCTAACCATGCTTCGA	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	369-1157 bp	1.0	87.2
ndhE-ndhG_p2	TGCTCTAACCATGCTTCGAC	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	368-1156 bp	1.0	87.2
ndhE-ndhG_p3	AGTGCTCTAACCATGCTTCG	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	370-1158 bp	1.0	87.2
ndhE-ndhG_p4	AACCATGCTTCGACTTGTGA	AGTTGCCTTGATAGGTGCAA	45.0	45.0	57.7	57.4	362-1150 bp	1.0	85.3
ndhE-ndhG_p5	GCTCTAACCATGCTTCGACT	AGTTGCCTTGATAGGTGCAA	50.0	45.0	57.7	57.4	367-1155 bp	1.0	84.9