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psbE-petL

LSC · 74958-76665 · 1708 bp

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Pi 0.0445

MarkerSeek score 85.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1708
variable_sites: 209
pi: 0.0445
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACTGACGAATAACCAACCCG	GCGCTGGGGTTATAGTTGAA	50.0	50.0	57.9	58.0	1402-2513 bp	1.0	91.0
psbE-petL_p2	ACTGACGAATAACCAACCCG	AAGCGCTGGGGTTATAGTTG	50.0	50.0	57.9	58.0	1404-2515 bp	1.0	91.0
psbE-petL_p3	ACTGACGAATAACCAACCCG	AATCAAAAGCGCTGGGGTTA	50.0	45.0	57.9	58.1	1410-2521 bp	1.0	90.6
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GCGCTGGGGTTATAGTTGAA	45.0	50.0	58.2	58.0	1398-2509 bp	1.0	90.4
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCGCTGGGGTTATAGTTG	45.0	50.0	58.2	58.0	1400-2511 bp	1.0	90.3