Hotspot

atpB-rbcL

LSC · 62260-63353 · 1094 bp

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Pi 0.0673

MarkerSeek score 82.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 177
pi: 0.0673
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGAGCAATACGCCCTAGG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	872-1417 bp	1.0	88.4
atpB-rbcL_p2	GAGCAATACGCCCTAGGTTT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	869-1414 bp	1.0	88.4
atpB-rbcL_p3	TTTGAGCAATACGCCCTAGG	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.7	58.4	873-1418 bp	1.0	86.0
atpB-rbcL_p4	GAGCAATACGCCCTAGGTTT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.7	58.4	870-1415 bp	1.0	86.0
atpB-rbcL_p5	TCTTCAAGTGTGGAAACCGC	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.7	58.1	846-1391 bp	1.0	85.5